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08cefec
NF_MAAffymetrix: update qmd structure
cyouh95 Dec 1, 2024
501ec0f
NF_MAAffymetrix: #113 update handling custom annotations
cyouh95 Jan 5, 2025
475a337
NF_MAAffymetrix: update pipeline documentation
cyouh95 Jan 5, 2025
6249c40
NF_MAAffymetrix: move updated doc to new pipeline version
cyouh95 Jan 10, 2025
683e0c3
NF_MAAffymetrix: track array annotations
cyouh95 Jan 19, 2025
8e6a96a
NF_MAAffymetrix: #113 update custom annotations config
cyouh95 Jan 20, 2025
4b490ba
NF_MAAffymetrix: use reference annotations GL-DPPD-7110-A
cyouh95 Jan 20, 2025
645b258
NF_MAAffymetrix: update tool versions
cyouh95 Jan 20, 2025
7b14d1d
NF_MAAffymetrix: update pipeline version from GL-DPPD-7114 to GL-DPPD…
cyouh95 Jan 21, 2025
a177711
NF_MAAffymetrix: update pipeline doc
cyouh95 Jan 31, 2025
0cd2a59
NF_MAAffymetrix: update pipeline doc
cyouh95 Feb 4, 2025
cfb2e94
Update GL-DPPD-7114-A.md
asaravia-butler Feb 7, 2025
7433f69
NF_MAAffymetrix: reorder DE table columns
cyouh95 Feb 10, 2025
44ecb25
NF_MAAffymetrix: remove visualization_PCA_table_GLmicroarray.csv output
cyouh95 Feb 10, 2025
bfa4eba
NF_MAAffymetrix: update report headings
cyouh95 Feb 10, 2025
a228c94
NF_MAAffymetrix: remove viz output from V&V
cyouh95 Feb 10, 2025
d07ee40
NF_MAAffymetrix: use original sample name in output files
cyouh95 Feb 10, 2025
f8bb3fe
Revert "NF_MAAffymetrix: use original sample name in output files"
cyouh95 Feb 11, 2025
2529319
NF_MAAffymetrix: minor updates to qmd
cyouh95 Feb 27, 2025
3c8e0af
NF_MAAffymetrix: update accepted ISA field name for label
cyouh95 Mar 4, 2025
57afe18
NF_MAAffymetrix: minor updates to workflow version 1.0.5
cyouh95 Mar 4, 2025
cca1601
NF_MAAffymetrix: minor update to pipeline doc
cyouh95 Mar 4, 2025
7733b7c
NF_MAAffymetrix: update custom functions in pipeline doc
cyouh95 Mar 25, 2025
0d79447
NF_MAAffymetrix: update nextflow version from 23.10.1 to 24.10.5
cyouh95 Mar 25, 2025
93d32ee
Update pipeline and annotation README docs
bnovak32 May 28, 2025
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array_design,annot_type,annot_filename,download_link,download_date
AFFY E coli Genome 2 0,3prime-IVT,E_coli_2.na36.annot.csv,https://www.thermofisher.com/order/catalog/product/sec/assets?url=TFS-Assets/LSG/Support-Files/E_coli_2-na36-annot-csv.zip,2024-06-15
AFFY GeneChip P. aeruginosa Genome,3prime-IVT,Pae_G1a.na36.annot.csv,https://www.thermofisher.com/order/catalog/product/sec/assets?url=TFS-Assets/LSG/Support-Files/Pae_G1a-na36-annot-csv.zip,2024-06-15
1,616 changes: 1,616 additions & 0 deletions Microarray/Affymetrix/Pipeline_GL-DPPD-7114_Versions/GL-DPPD-7114-A.md

Large diffs are not rendered by default.

6 changes: 5 additions & 1 deletion Microarray/Affymetrix/README.md
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# GeneLab bioinformatics processing pipeline for Affymetrix microarray data


> **The document [`GL-DPPD-7114.md`](Pipeline_GL-DPPD-7114_Versions/GL-DPPD-7114.md) holds an overview and example commands for how GeneLab processes Affymetrix microarray datasets. See the [Repository Links](#repository-links) descriptions below for more information. Processed data output files and processing code is provided for each GLDS dataset along with the processed data in the [Open Science Data Repository (OSDR)](https://osdr.nasa.gov/bio/repo/).**
> **The document [`GL-DPPD-7114-A.md`](Pipeline_GL-DPPD-7114_Versions/GL-DPPD-7114-A.md) holds an overview and example commands for how GeneLab processes Affymetrix microarray datasets. See the [Repository Links](#repository-links) descriptions below for more information. Processed data output files and processing code is provided for each GLDS dataset along with the processed data in the [Open Science Data Repository (OSDR)](https://osdr.nasa.gov/bio/repo/).**

---

Expand All @@ -20,6 +20,10 @@

- Contains instructions for installing and running the GeneLab NF_MAAffymetrix workflow

* [**Array_Annotations**](Array_Annotations)

- Contains the custom annotations table used in the GeneLab NF_MAAffymetrix

---
**Developed by:**
Jonathan Oribello
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Expand Up @@ -5,11 +5,11 @@ All notable changes to this project will be documented in this file.
The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/),
and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).

## [1.0.5](https://github.com/nasa/GeneLab_Data_Processing/tree/NF_MAAffymetrix_1.0.5/Microarray/Affymetrix/Workflow_Documentation/NF_MAAffymetrix) - 2024-08-30
## [1.0.5](https://github.com/nasa/GeneLab_Data_Processing/tree/NF_MAAffymetrix_1.0.5/Microarray/Affymetrix/Workflow_Documentation/NF_MAAffymetrix) - 2025-03-03

### Added

- Add support for bacteria annotations using manufacturer annotations ([#113](https://github.com/nasa/GeneLab_Data_Processing/issues/113))
- Support for custom annotations, see [specification](examples/annotations/README.md) ([#113](https://github.com/nasa/GeneLab_Data_Processing/issues/113))
- Add option to skip differential expression analysis (`--skipDE`) ([#104](https://github.com/nasa/GeneLab_Data_Processing/issues/104))

### Changed
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Expand Up @@ -4,7 +4,7 @@

### Implementation Tools <!-- omit in toc -->

The current GeneLab Affymetrix Microarray consensus processing pipeline (NF_MAAffymetrix), [GL-DPPD-7114](../../Pipeline_GL-DPPD-7114_Versions/GL-DPPD-7114.md), is implemented as a [Nextflow](https://nextflow.io/) DSL2 workflow and utilizes [Singularity](https://docs.sylabs.io/guides/3.10/user-guide/introduction.html) to run all tools in containers. This workflow (NF_MAAffymetrix) is run using the command line interface (CLI) of any unix-based system. While knowledge of creating workflows in Nextflow is not required to run the workflow as is, [the Nextflow documentation](https://nextflow.io/docs/latest/index.html) is a useful resource for users who want to modify and/or extend this workflow.
The current GeneLab Affymetrix Microarray consensus processing pipeline (NF_MAAffymetrix), [GL-DPPD-7114-A](../../Pipeline_GL-DPPD-7114_Versions/GL-DPPD-7114-A.md), is implemented as a [Nextflow](https://nextflow.io/) DSL2 workflow and utilizes [Singularity](https://docs.sylabs.io/guides/3.10/user-guide/introduction.html) to run all tools in containers. This workflow (NF_MAAffymetrix) is run using the command line interface (CLI) of any unix-based system. While knowledge of creating workflows in Nextflow is not required to run the workflow as is, [the Nextflow documentation](https://nextflow.io/docs/latest/index.html) is a useful resource for users who want to modify and/or extend this workflow.

### Workflow & Subworkflows <!-- omit in toc -->

Expand All @@ -14,8 +14,8 @@ The current GeneLab Affymetrix Microarray consensus processing pipeline (NF_MAAf

---
The NF_MAAffymetrix workflow is composed of three subworkflows as shown in the image above.
Below is a description of each subworkflow and the additional output files generated that are not already indicated in the [GL-DPPD-7114 pipeline
document](../../Pipeline_GL-DPPD-7114_Versions/GL-DPPD-7114.md):
Below is a description of each subworkflow and the additional output files generated that are not already indicated in the [GL-DPPD-7114-A pipeline
document](../../Pipeline_GL-DPPD-7114_Versions/GL-DPPD-7114-A.md):

1. **Analysis Staging Subworkflow**

Expand All @@ -26,7 +26,7 @@ document](../../Pipeline_GL-DPPD-7114_Versions/GL-DPPD-7114.md):
2. **Affymetrix Microarray Processing Subworkflow**

- Description:
- This subworkflow uses the staged raw data and metadata parameters from the Analysis Staging Subworkflow to generate processed data using the [GL-DPPD-7114 pipeline](../../Pipeline_GL-DPPD-7114_Versions/GL-DPPD-7114.md).
- This subworkflow uses the staged raw data and metadata parameters from the Analysis Staging Subworkflow to generate processed data using the [GL-DPPD-7114-A pipeline](../../Pipeline_GL-DPPD-7114_Versions/GL-DPPD-7114-A.md).

1. **V&V Pipeline Subworkflow**

Expand Down Expand Up @@ -200,7 +200,7 @@ All R code steps and output are rendered within a Quarto document yielding the f


The outputs from the Analysis Staging and V&V Pipeline Subworkflows are described below:
> Note: The outputs from the Affymetrix Microarray Processing Subworkflow are documented in the [GL-DPPD-7114.md](../../../Pipeline_GL-DPPD-7114_Versions/GL-DPPD-7114.md) processing protocol.
> Note: The outputs from the Affymetrix Microarray Processing Subworkflow are documented in the [GL-DPPD-7114-A.md](../../../Pipeline_GL-DPPD-7114_Versions/GL-DPPD-7114-A.md) processing protocol.

**Analysis Staging Subworkflow**

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# Custom Annotations Specification

## Description

* If using custom gene annotations when processing Affymetrix datasets through GeneLab's Affymetrix processing pipeline, a csv config file must be provided as specified below.
* See [Affymetrix_array_annotations.csv](https://github.com/nasa/GeneLab_Data_Processing/tree/master/Microarray/Affymetrix/Array_Annotations/Affymetrix_array_annotations.csv)
for the latest config file used at GeneLab.


## Example

- [config.csv](config.csv)


## Required columns

| Column Name | Type | Description | Example |
|:------------|:-----|:------------|:--------|
| array_design | string | A bioMart attribute identifier denoting the microarray probe/probeset attribute used for annotation mapping. | AFFY E coli Genome 2 0 |
| annot_type | string | Used to determine how the custom annotations are parsed before merging to the data. Currently, only the below are supported: <ul><li>`3prime-IVT`: Annotations file is expected to be in the format of the 3' IVT expression analysis arrays annotations by [Thermo Fisher](https://www.thermofisher.com/us/en/home/life-science/microarray-analysis/microarray-data-analysis/genechip-array-annotation-files.html)</li><li>`custom`: Annotations file is merged as is, expected to have the following columns: `ProbesetID`, `ENTREZID`, `SYMBOL`, `GENENAME`, `ENSEMBL`, `REFSEQ`, `GOSLIM_IDS`, `STRING_id`, `count_gene_mappings`, `gene_mapping_source`</li></ul> | 3prime-IVT |
| annot_filename | string | Name of the custom annotations file. | E_coli_2.na36.annot.csv |

## Optional columns
If the file was downloaded from a website, provide the download link used and date
downloaded in additional columns after the required column for traceability.

| Column Name | Type | Description | Example |
|:------------|:-----|:------------|:--------|
| download_link | string | The URL used to retrieve the annotation file. | https://www.thermofisher.com/order/catalog/product/sec/assets?url=TFS-Assets/LSG/Support-Files/E_coli_2-na36-annot-csv.zip |
| download_date | date string | The date the file was retrieved in YYYY-MM-DD format. | 2024-06-15 |
Original file line number Diff line number Diff line change
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array_design,annot_type,annot_filename
AFFY E coli Genome 2 0,3prime-IVT,E_coli_2.na36.annot.csv
AFFY GeneChip P. aeruginosa Genome,3prime-IVT,Pae_G1a.na36.annot.csv
Original file line number Diff line number Diff line change
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Sample Name,Study Assay Measurement,Study Assay Technology Type,Study Assay Technology Platform,organism,biomart_attribute,Source Name,Label,Array Data File Name,Array Data File Path,Comment[Array Data File Name],Factor Value[Spaceflight],Factor Value[Altered Gravity],Original Sample Name
Atha_Col-0_clsCC_FLT_1G_Rep1,transcription profiling,DNA microarray,Affymetrix,Arabidopsis thaliana,AFFY ATH1 121501,Culture cells_1,biotin,GLDS-213_microarray_FC_front.CEL.gz,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-213/download?source=datamanager&file=GLDS-213_microarray_FC_front.CEL.gz,GLDS-213_microarray_FC_front.CEL.gz,Space Flight,1G by centrifugation,Atha_Col-0_clsCC_FLT_1G_Rep1
Atha_Col-0_clsCC_FLT_1G_Rep2,transcription profiling,DNA microarray,Affymetrix,Arabidopsis thaliana,AFFY ATH1 121501,Culture cells_2,biotin,GLDS-213_microarray_FC_rear.CEL.gz,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-213/download?source=datamanager&file=GLDS-213_microarray_FC_rear.CEL.gz,GLDS-213_microarray_FC_rear.CEL.gz,Space Flight,1G by centrifugation,Atha_Col-0_clsCC_FLT_1G_Rep2
Atha_Col-0_clsCC_FLT_uG_Rep1,transcription profiling,DNA microarray,Affymetrix,Arabidopsis thaliana,AFFY ATH1 121501,Culture cells_3,biotin,GLDS-213_microarray_FS_front.CEL.gz,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-213/download?source=datamanager&file=GLDS-213_microarray_FS_front.CEL.gz,GLDS-213_microarray_FS_front.CEL.gz,Space Flight,uG,Atha_Col-0_clsCC_FLT_uG_Rep1
Atha_Col-0_clsCC_FLT_uG_Rep2,transcription profiling,DNA microarray,Affymetrix,Arabidopsis thaliana,AFFY ATH1 121501,Culture cells_4,biotin,GLDS-213_microarray_FS_rear.CEL.gz,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-213/download?source=datamanager&file=GLDS-213_microarray_FS_rear.CEL.gz,GLDS-213_microarray_FS_rear.CEL.gz,Space Flight,uG,Atha_Col-0_clsCC_FLT_uG_Rep2
Atha_Col-0_clsCC_GC_1G_Rep1,transcription profiling,DNA microarray,Affymetrix,Arabidopsis thaliana,AFFY ATH1 121501,Culture cells_5,biotin,GLDS-213_microarray_GS_front.CEL.gz,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-213/download?source=datamanager&file=GLDS-213_microarray_GS_front.CEL.gz,GLDS-213_microarray_GS_front.CEL.gz,Ground Control,1G on Earth,Atha_Col-0_clsCC_GC_1G_Rep1
Atha_Col-0_clsCC_GC_1G_Rep2,transcription profiling,DNA microarray,Affymetrix,Arabidopsis thaliana,AFFY ATH1 121501,Culture cells_6,biotin,GLDS-213_microarray_GS_rear.CEL.gz,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-213/download?source=datamanager&file=GLDS-213_microarray_GS_rear.CEL.gz,GLDS-213_microarray_GS_rear.CEL.gz,Ground Control,1G on Earth,Atha_Col-0_clsCC_GC_1G_Rep2
Original file line number Diff line number Diff line change
@@ -0,0 +1,19 @@
Sample Name,Study Assay Measurement,Study Assay Technology Type,Study Assay Technology Platform,organism,biomart_attribute,Source Name,Label,Array Data File Name,Array Data File Path,Comment[Array Data File Name],Factor Value[Developmental Stage],Factor Value[Spaceflight],Original Sample Name
Dmel_Hml-GAL4-UAS-GFP_wo_FLT_3rd-Instar-Larva_Rep1,transcription profiling,DNA microarray,Affymetrix,Drosophila melanogaster,AFFY Drosophila 2,GSM588931 1,biotin,GLDS-3_microarray_GSM588948.CEL,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-3/download?source=datamanager&file=GLDS-3_microarray_GSM588948.CEL,GLDS-3_microarray_GSM588948.CEL,third instar larva stage,Space Flight,Dmel_Hml-GAL4-UAS-GFP_wo_FLT_3rd-Instar-Larva_Rep1
Dmel_Hml-GAL4-UAS-GFP_wo_FLT_3rd-Instar-Larva_Rep2,transcription profiling,DNA microarray,Affymetrix,Drosophila melanogaster,AFFY Drosophila 2,GSM588932 1,biotin,GLDS-3_microarray_GSM588947.CEL,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-3/download?source=datamanager&file=GLDS-3_microarray_GSM588947.CEL,GLDS-3_microarray_GSM588947.CEL,third instar larva stage,Space Flight,Dmel_Hml-GAL4-UAS-GFP_wo_FLT_3rd-Instar-Larva_Rep2
Dmel_Hml-GAL4-UAS-GFP_wo_FLT_3rd-Instar-Larva_Rep3,transcription profiling,DNA microarray,Affymetrix,Drosophila melanogaster,AFFY Drosophila 2,GSM588933 1,biotin,GLDS-3_microarray_GSM588946.CEL,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-3/download?source=datamanager&file=GLDS-3_microarray_GSM588946.CEL,GLDS-3_microarray_GSM588946.CEL,third instar larva stage,Space Flight,Dmel_Hml-GAL4-UAS-GFP_wo_FLT_3rd-Instar-Larva_Rep3
Dmel_Hml-GAL4-UAS-GFP_wo_FLT_3rd-Instar-Larva_Rep4,transcription profiling,DNA microarray,Affymetrix,Drosophila melanogaster,AFFY Drosophila 2,GSM588934 1,biotin,GLDS-3_microarray_GSM588945.CEL,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-3/download?source=datamanager&file=GLDS-3_microarray_GSM588945.CEL,GLDS-3_microarray_GSM588945.CEL,third instar larva stage,Space Flight,Dmel_Hml-GAL4-UAS-GFP_wo_FLT_3rd-Instar-Larva_Rep4
Dmel_Hml-GAL4-UAS-GFP_wo_FLT_3rd-Instar-Larva_Rep5,transcription profiling,DNA microarray,Affymetrix,Drosophila melanogaster,AFFY Drosophila 2,GSM588935 1,biotin,GLDS-3_microarray_GSM588944.CEL,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-3/download?source=datamanager&file=GLDS-3_microarray_GSM588944.CEL,GLDS-3_microarray_GSM588944.CEL,third instar larva stage,Space Flight,Dmel_Hml-GAL4-UAS-GFP_wo_FLT_3rd-Instar-Larva_Rep5
Dmel_Hml-GAL4-UAS-GFP_wo_FLT_3rd-Instar-Larva_Rep6,transcription profiling,DNA microarray,Affymetrix,Drosophila melanogaster,AFFY Drosophila 2,GSM588936 1,biotin,GLDS-3_microarray_GSM588943.CEL,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-3/download?source=datamanager&file=GLDS-3_microarray_GSM588943.CEL,GLDS-3_microarray_GSM588943.CEL,third instar larva stage,Space Flight,Dmel_Hml-GAL4-UAS-GFP_wo_FLT_3rd-Instar-Larva_Rep6
Dmel_Hml-GAL4-UAS-GFP_wo_FLT_Adult_Rep1,transcription profiling,DNA microarray,Affymetrix,Drosophila melanogaster,AFFY Drosophila 2,GSM588937 1,biotin,GLDS-3_microarray_GSM588942.CEL,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-3/download?source=datamanager&file=GLDS-3_microarray_GSM588942.CEL,GLDS-3_microarray_GSM588942.CEL,adult,Space Flight,Dmel_Hml-GAL4-UAS-GFP_wo_FLT_Adult_Rep1
Dmel_Hml-GAL4-UAS-GFP_wo_FLT_Adult_Rep2,transcription profiling,DNA microarray,Affymetrix,Drosophila melanogaster,AFFY Drosophila 2,GSM588938 1,biotin,GLDS-3_microarray_GSM588941.CEL,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-3/download?source=datamanager&file=GLDS-3_microarray_GSM588941.CEL,GLDS-3_microarray_GSM588941.CEL,adult,Space Flight,Dmel_Hml-GAL4-UAS-GFP_wo_FLT_Adult_Rep2
Dmel_Hml-GAL4-UAS-GFP_wo_FLT_Adult_Rep3,transcription profiling,DNA microarray,Affymetrix,Drosophila melanogaster,AFFY Drosophila 2,GSM588939 1,biotin,GLDS-3_microarray_GSM588940.CEL,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-3/download?source=datamanager&file=GLDS-3_microarray_GSM588940.CEL,GLDS-3_microarray_GSM588940.CEL,adult,Space Flight,Dmel_Hml-GAL4-UAS-GFP_wo_FLT_Adult_Rep3
Dmel_Hml-GAL4-UAS-GFP_wo_GC_3rd-Instar-Larva_Rep1,transcription profiling,DNA microarray,Affymetrix,Drosophila melanogaster,AFFY Drosophila 2,GSM588940 1,biotin,GLDS-3_microarray_GSM588939.CEL,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-3/download?source=datamanager&file=GLDS-3_microarray_GSM588939.CEL,GLDS-3_microarray_GSM588939.CEL,third instar larva stage,Ground Control,Dmel_Hml-GAL4-UAS-GFP_wo_GC_3rd-Instar-Larva_Rep1
Dmel_Hml-GAL4-UAS-GFP_wo_GC_3rd-Instar-Larva_Rep2,transcription profiling,DNA microarray,Affymetrix,Drosophila melanogaster,AFFY Drosophila 2,GSM588941 1,biotin,GLDS-3_microarray_GSM588938.CEL,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-3/download?source=datamanager&file=GLDS-3_microarray_GSM588938.CEL,GLDS-3_microarray_GSM588938.CEL,third instar larva stage,Ground Control,Dmel_Hml-GAL4-UAS-GFP_wo_GC_3rd-Instar-Larva_Rep2
Dmel_Hml-GAL4-UAS-GFP_wo_GC_3rd-Instar-Larva_Rep3,transcription profiling,DNA microarray,Affymetrix,Drosophila melanogaster,AFFY Drosophila 2,GSM588942 1,biotin,GLDS-3_microarray_GSM588937.CEL,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-3/download?source=datamanager&file=GLDS-3_microarray_GSM588937.CEL,GLDS-3_microarray_GSM588937.CEL,third instar larva stage,Ground Control,Dmel_Hml-GAL4-UAS-GFP_wo_GC_3rd-Instar-Larva_Rep3
Dmel_Hml-GAL4-UAS-GFP_wo_GC_3rd-Instar-Larva_Rep4,transcription profiling,DNA microarray,Affymetrix,Drosophila melanogaster,AFFY Drosophila 2,GSM588943 1,biotin,GLDS-3_microarray_GSM588936.CEL,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-3/download?source=datamanager&file=GLDS-3_microarray_GSM588936.CEL,GLDS-3_microarray_GSM588936.CEL,third instar larva stage,Ground Control,Dmel_Hml-GAL4-UAS-GFP_wo_GC_3rd-Instar-Larva_Rep4
Dmel_Hml-GAL4-UAS-GFP_wo_GC_3rd-Instar-Larva_Rep5,transcription profiling,DNA microarray,Affymetrix,Drosophila melanogaster,AFFY Drosophila 2,GSM588944 1,biotin,GLDS-3_microarray_GSM588935.CEL,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-3/download?source=datamanager&file=GLDS-3_microarray_GSM588935.CEL,GLDS-3_microarray_GSM588935.CEL,third instar larva stage,Ground Control,Dmel_Hml-GAL4-UAS-GFP_wo_GC_3rd-Instar-Larva_Rep5
Dmel_Hml-GAL4-UAS-GFP_wo_GC_3rd-Instar-Larva_Rep6,transcription profiling,DNA microarray,Affymetrix,Drosophila melanogaster,AFFY Drosophila 2,GSM588945 1,biotin,GLDS-3_microarray_GSM588934.CEL,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-3/download?source=datamanager&file=GLDS-3_microarray_GSM588934.CEL,GLDS-3_microarray_GSM588934.CEL,third instar larva stage,Ground Control,Dmel_Hml-GAL4-UAS-GFP_wo_GC_3rd-Instar-Larva_Rep6
Dmel_Hml-GAL4-UAS-GFP_wo_GC_Adult_Rep1,transcription profiling,DNA microarray,Affymetrix,Drosophila melanogaster,AFFY Drosophila 2,GSM588946 1,biotin,GLDS-3_microarray_GSM588933.CEL,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-3/download?source=datamanager&file=GLDS-3_microarray_GSM588933.CEL,GLDS-3_microarray_GSM588933.CEL,adult,Ground Control,Dmel_Hml-GAL4-UAS-GFP_wo_GC_Adult_Rep1
Dmel_Hml-GAL4-UAS-GFP_wo_GC_Adult_Rep2,transcription profiling,DNA microarray,Affymetrix,Drosophila melanogaster,AFFY Drosophila 2,GSM588947 1,biotin,GLDS-3_microarray_GSM588932.CEL,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-3/download?source=datamanager&file=GLDS-3_microarray_GSM588932.CEL,GLDS-3_microarray_GSM588932.CEL,adult,Ground Control,Dmel_Hml-GAL4-UAS-GFP_wo_GC_Adult_Rep2
Dmel_Hml-GAL4-UAS-GFP_wo_GC_Adult_Rep3,transcription profiling,DNA microarray,Affymetrix,Drosophila melanogaster,AFFY Drosophila 2,GSM588948 1,biotin,GLDS-3_microarray_GSM588931.CEL,https://genelab-data.ndc.nasa.gov/geode-py/ws/studies/OSD-3/download?source=datamanager&file=GLDS-3_microarray_GSM588931.CEL,GLDS-3_microarray_GSM588931.CEL,adult,Ground Control,Dmel_Hml-GAL4-UAS-GFP_wo_GC_Adult_Rep3
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